This project belongs to the field of clinical application and basic research on internal medicine in traditional Chinese medicine. Alcohol poisoning is a major public health problem in today's world. In recent years, with the improvement of people's living standards and the increase in the number of drinkers in China, alcoholic liver disease (ALD) has become the second largest liver disease after viral hepatitis. Research on the prevention and treatment of ALD by traditional Chinese medicine started relatively late, and there are still significant controversies over its core pathogenesis and the distribution of syndromes. The targeted use of empirical medicine is still lacking, and a stable and reliable animal model has not yet been established, which has become a 'bottleneck' restricting the in-depth research on the prevention and treatment of ALD by traditional Chinese medicine. In the early stage of the project, a theory of pathogenesis for ALD based on clinical epidemiology and efficacy evidence was established, and an effective prescription for treating ALD, Qinggan Huoxue Formula, was developed. The clinical efficacy of Qinggan Huoxue Formula has been confirmed through RCT research. Under the guidance of the theory of 'Combining Disease Patterns with Syndrome Patterns', this project has achieved the following main scientific findings: (1) Starting from the key pathological and physiological mechanism of ALD, Kupffer cells-lipopolysaccharide (KC-LPS), through biochemical, immunological, pathological, molecular biological methods, etc., we have demonstrated the pathophysiological basis of syndrome patterns for the pathogenesis of ALD's 'stasis heat' pattern. We have found that CD14 overexpression is characterized by a 'heat heavier than stasis', while Toll-like receptor 4 (TLR4) overexpression shows a regular change of 'stasis heavier than heat'. This further establishes the role of the 'stasis heat' pathogenesis theory in the onset and prevention of ALD. (2) Preparation of an animal model of natural progression of ALD to alcoholic liver fibrosis (AF). Evidence has been found that uPA/PAI-1 is involved in the formation and development of atrial fibrillation (AF), and gene silencing technology has confirmed that PAI-1 plays a key role in AF formation, leading to new discoveries in the pathophysiology of ALD. (3) For the first time, active components in Qinggan Huoxue Prescription were discovered through both in vitro and in vivo metabolism studies. The effects of these components on hepatic stellate cells (HSC) and RNAi-HSC expression of uPA, PAI-1, and MMPs were investigated. It was proposed that salvianolic acid B is the main active component, and PAI-1 is its main pharmacological target, reflecting the systematic and comprehensive nature of this project's research. This project has received 2 National Natural Science Foundation grants and 1 Ministry of Education New Century Excellent Talents Fund, with a total of 27 papers published, including 3 SCI-indexed articles with 15 citations each. One postdoctoral fellow, one doctoral student, and four master's students have been trained. The project team was responsible for drafting the The 'Guidelines for the Diagnosis and Treatment of Alcoholic Liver Disease with Traditional Chinese Medicine' have become an industry standard ('Guidelines for the Diagnosis and Treatment of Common Diseases in Internal Medicine of Traditional Chinese Medicine', published by the Chinese Association of Traditional Chinese Medicine, 2008), and the research results were selected for inclusion in the medical publication 'Fatty Liver Disease' (edited by Fan Jiangao, second edition, People's Health Publishing House, March 2013). The completion of the project has played a key role in enriching the theory of 'stasis-heat' pathogenesis and improving the clinical efficacy and academic level of traditional Chinese medicine in the prevention and treatment of ALD.